ELISA介绍

技术原理:

酶联免疫吸附试验(ELISA)是一种实验室常用的检测方法, 广泛应用于测量溶液中的分析物(通常是抗体或抗原)的浓度。与其他基于抗体的检测方法不同的是, 酶联免疫吸附实验(ELISA)或酶免疫测定(EIA)通过与固相支持物(通常为聚乙烯多孔板)的结合和系列洗涤步骤, 实现特异性和非特异性相互作用的分离, 并可通过有色产物的形成, 定量分析原始样品中分析物的含量。

ELISA实验快速且简单, 可迅速分析大量平行的样品, 在基础研究、临床前研究和临床诊断实践中广泛使用。

ELISA包括直接法ELISA间接法ELISA竞争法ELISA夹心法ELISA

双抗体夹心法ELISA是最常见的ELISA类型。使用两种特异性抗体将抗原夹在中间,通常称为抗体对。将捕获抗体包被在微孔板上,加入样品,目标蛋白与抗体结合并固定在板上。然后加入偶联的检测抗体,并与靶蛋白上的其它表位结合。加入底物并产生与样品中目标分析物量成比例的信号。双抗体夹心法ELISA需要两种抗体才能与目标蛋白结合,具有高度特异性。

优点:

特异性高,使用的捕获抗体和检测抗体都是检测物特异性的;

灵敏度高,酶标二抗放大检测信号;

适用于复杂样品, 抗原不需进行纯化即可用于检测;

缺点:

适用于检测分子中具有至少两个抗原决定簇的多价抗原;

不能用于小分子半抗原的检测;

双抗体夹心法ELISA原理图

四正柏ELISA试剂盒

四正柏一直专注于单克隆抗体和细胞因子检测试剂的开发和生产。ELISA试剂盒已有30年研发工艺积累,确保试剂盒重复性佳、灵敏度高、特异性强、准确度高、回收率好。

提供经过国际标准质控的400多种ELISA试剂盒,能检测人、小鼠、大鼠、猪等多种种属细胞因子,研究领域覆盖免疫学、肿瘤学、神经生物学、发育生物学、氧化应激等,以质优价美的特色畅销国内外市场。除了提供成品试剂盒外,也可以提供定制ELISA试剂盒约150多种。

试剂盒样本类型多样,除了血清、血浆、细胞培养上清外,多种组织匀浆和体液均有文献发表。

目前有1000多篇SCI文献发表,试剂盒也以其高质量发表在《Nature》、《Immunity》等知名期刊上,深受广大客户的一致好评和深度信赖!

ELISA热销指标
检测靶标 检测种属 灵敏度 检测范围 规格 产品编号 文献引用数
Mouse TNF-α (肿瘤坏死因子α) Mouse 7 pg/mL 15.6 -500 pg/mL 48/96 wells CME0004 132
Mouse IL-6 (白细胞介素6) Mouse 4 pg/mL 7.8 -500 pg/mL 48/96 wells CME0006 102
Mouse IL-1β (白细胞介素1β) Mouse 7 pg/mL 31.25-2000 pg/mL 48/96 wells CME0015 61
Human TNF-α (肿瘤坏死因子α) Human 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CHE0019 55
Human IL-1β (白细胞介素1β) Human 7 pg/mL 15.6 - 500 pg/mL 48/96 wells CHE0001 33
Human IL-6 (白细胞介素6) Human 2 pg/mL 3.9 - 250 pg/mL 48/96 wells CHE0009 52
Rat TNF-α (肿瘤坏死因子α) Rat 15 pg/mL 31.25-2,000 pg/mL 48/96 wells CRE0003 27
Mouse IFN-γ (γ干扰素) Mouse 4 pg/mL 7.8-500 pg/mL 48/96 wells CME0003 53
Mouse IL-10 (白细胞介素10) Mouse 7 pg/mL 15.6-1000 pg/mL 48/96 wells CME0016 21
Rat IL-1β (白细胞介素1β) Rat 15 pg/mL 62.5-4,000 pg/mL 48/96 wells CRE0006 9
Human IL-10 (白细胞介素10) Human 4 pg/mL 7.8 - 5,00 pg/mL 48/96 wells CHE0013 10
Rat IL-6 (白细胞介素6) Rat 31 pg/mL 62.5-4,000 pg/mL 48/96 wells CRE0005 21
Human TGF-β1 (转化生长因子β1) Human 31 pg/mL 62.5-4,000 pg/mL 48/96 wells CHE0029 8
Human IL-2 (白细胞介素2) Human 4 pg/mL 7.8 - 500 pg/mL 48/96 wells CHE0003 9
Human IFN-β(人β干扰素) Human 4 pg/mL 7.8 - 500 pg/mL 48/96 wells CHE0274 6
Mouse IL-4 (白细胞介素4) Mouse 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CME0005 20
Human IL-8 (白细胞介素8) Human 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CHE0011 26
Mouse IL-8/CXCL1/CXCL1 (白细胞介素8) Mouse 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CME0008 5
Human IL-17 (白细胞介素17) Human 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CHE0054 5
Human IL-18 (白细胞介素18) Human 20 pg/mL 39 - 2,500 pg/mL 48/96 wells CHE0007 3
Human VEGF (血管内皮生长因子) Human 7 pg/mL 31.25-2,000 pg/mL 48/96 wells CHE0043 17
Mouse TGF-β1 (转化生长因子β1) Mouse 7 pg/mL 15.6-2,000 pg/mL 48/96 wells CME0020 14
Human IL-4 (白细胞介素4) Human 7 pg/mL 15.6 - 500 pg/mL 48/96 wells CHE0005 6
Mouse IFN-β(小鼠β干扰素) Mouse 6 pg/mL 12.5 - 800 pg/mL 48/96 wells CME0116 6
Human MCP-1 (单核细胞趋化蛋白1) Human 7 pg/mL 15.6-1,000 pg/mL 48/96 wells CHE0103 7
Mouse IL-17A (白细胞介素17A) Mouse 4 pg/mL 7.8 - 500 pg/mL 48/96 wells CME0041 7
其他指标
Human
Activin A CXCL11/I-TAC Granzyme A IL-24/MDA-7 MMP-13 sE-selectin
ADAMTS13 CXCL12/SDF-1 Granzyme B IL-27 MMP-2 sFRP-5
ADAMTS4 CXCL13/BCA-1/BLC HB-EGF IL-28/IFN-λ2 MMP-3 sICAM-1
ADAMTS5 CXCL14/BRAK Hepcidin IL-29/IFN-λ1 MMP-9 sIL-2R
Adiponectin CXCL16 HGF IL-3 MyD88 Stanniocalcin 1
Amphiregulin CXCL2/GRO β HSP60 IL-31 Myeloperoxidase sTNF RI/TNFRSF1A
Angiopoietin-2 CXCL4/PF4 HSP70/HSPA1A IL-32 α NT-ProANP sTNF RII/TNFRSF1B
Angiopoietin-like 4 CXCL5/ENA-78 Indoleamine 2,3-dioxygenase/IDO IL-33 OPG Syndecan-1
Apolipoprotein A-1/ApoA1 CXCL6/GCP-2 IFN-α IL-34 OSM-R TGF-α
BAFF/TNFSF13B CXCL7/NAP-2 IgA IL-36 γ/IL-1F9 Osteepontin OPN TGF-β3
BDNF Cytochrome C IgE IL-37/IL-1F7 Park7/DJ-1 Thrombomodulin/BDCA-3
BMP-2 DDR1 IGFBP-1 IL-5 PBEF/Visfatin Thrombopoietin
BMP-6 DPPIV/CD26 IGFBP-7/IGFBP-rp1 IL-6 Rα PD-1 Thrombospondin-1
BMP-7 EDIL3 IGF-I IL-9 PDGF-AA TIM-1/KIM-1
CCL1/I-309 EGF IgG total Irisin/FNDC5 PDGF-AB TIMP-1
CCL17/TARC eNOS IgM KGF/FGF-7 PDGF-BB TIMP-2
CCL19/MIP-3 β Fas Ligand/TNFSF6 IL-1 R4/ST2 Ki-67/MKI67 PDGF-DD TL1A/TNFSFS15
CCL21 Fas/Apo-1 IL-11 Klotho Periostin/OSF-2 TLR2
CCL22/MDC Fetuin A/AHSG IL-12p40 LAG-3 Peroxiredoxin 2 TNF-β
CCL24/Eotaxin-2 FGF acidic IL-12p70 Leptin PIGF Total MMP-1
CCL25/TECK FGF basic IL-13 LIF Pro-Collagen I α1 TRAIL/TNFSF10
CCL26/Eotaxin-3 FGF-19 IL-15 lipocalin-2/NGAL Proinsulin TRANCE/RANK L/TNFSF11
CD27/TNFRSF7 FGF-21 IL-17C MCP-2/CCL8 Prolactin TSLP
Complement Component C5a FGF-23 IL-17E MCP-3 P-selectin Uromodulin
Complement Factor H Galectin-1 IL-18 BPa Elisa Kit M-CSF Rantes VCAM-1
Corin Galectin-3 IL-19 MICA RBP4 Vitamin D BP
CRP G-CSF IL-1α MICB Resistin vWF-A2
Cu/ZnSOD GDF-15 IL-21 MIF S100B α-Defensin 1
CXCL1/GRO GDNF IL-22 MIG/CXCL9 sCD40L β-NGF
CXCL10/IP10 GM-CSF IL-23 MIP-3α/CCL20 Serpin E1/PAI-1
Mouse
Adiponectin/Acrp30 CXCL15/Lungkine IgA IL-21 MIP-1 β/CCL4 Thrombopoietin
BAFF/BLyS/TNFSF13B CXCL7/Thymus Chemokine-1 IgE IL-22 MIP-1 α/CCL3 TIMP-1
BDNF CXCL9/MIG IGF-I IL-23 MIP-2/CXCL2 TL1A/TNFSF15
CCL11/Eotaxin-1 EGF IgG total IL-25/IL-17E MMP-3 TRANCE/RANKL/TNFSF11
CCL24/Eotaxin/MPIF-2 Erythropoietin IgM IL-27 MMP-9 TSLP
CCL5/Rantes E-Selectin/CD62E IL-12/23 IL-33 Myeloperxidase VCAM-1
Chemerin FGF basic IL-12p70 IL-5 Neutrophil Elastase/ELA2 VEGF
Complement Component C5a FGF-21 IL-13 IL-7 NGAL/Lipocalin-2 YM1/Chitinase 3-like 3
CRP G-CSF IL-15 IL-9 PD-1 α-Fetoprotein/AFP
CX3CL1/Fractalkine GDF-15 IL-17F Leptin PlGF-2
CXCL10/IP10 GM-CSF IL-1ra/IL-1F3 LIX Prolactin
CXCL11/I-TAC Granzyme B IL-1α MCP-1 sICAM-1
CXCL12/SDF-1 HGF IL-2 MCP-2/CCL8 Thrombomodulin/BDCA-3
Rat Swine Multispecific
CRP IL-12p40 TIMP-1 IFN-γ Glucagon
EGF IL-17A Total MMP-9 IL-10 Insulin
GM-CSF IL-1α VEGF IL-12/IL-23p40 PDGF-BB
ICAM-1/CD54 IL-2 β-NGF IL-1β Total HIF-1α
IFN-γ IL-4 IL-2 Total HSP70/HSPA1A
IGF-I MCP-1 IL-4
IgG total MIP-2 IL-6
IgM NGAL/Lipocalin-2 IL-8
IL-10 TNF-α
辅助试剂
ELISA 辅助试剂
SFQ012 包被液 SFQ008 浓缩洗涤液(20X)
SFQ007 标准品 SFQ005 生物素化抗体
SFQ028 封板膜 SFQ0102 生物素化抗体稀释液
SFQ011 封闭液 SFQ001 显色剂/单组份超灵敏TMB
SFQ027 高吸附酶标板 SFQ0101 样本稀释液
SFQ0103 酶结合物稀释液 SFQ009 终止液

文献引用(部分)

1. Huang B, Chen Z, ect. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019 Nov 14;179 (5):1160-1176.e24. (IF: 41.582)

引用产品: Mouse TNF-α、IL-1β and IL-6 ELISA Kits

2. Shanshan Liu, Chang Liu, Xiaoxi Lv, etc. Thechemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis. Immunity. Published 17 August. (IF: 31.745)

引用产品: Mouse CCL1/TCA-3 ELISA Kit

3. Li B, Ji P, Peng SY, Pan P, etc. Nitric Oxide Release Device for Remote-Controlled Cancer Therapy by Wireless Charging. Adv Mater. 2020 Apr;32(16):e2000376.(IF: 30.849)

引用产品: Mouse TNF-α and IL-6 ELISA Kits

4. Zou MZ, Liu WL,etc. Artificial Natural Killer Cells for Specific Tumor Inhibition and Renegade Macrophage Re-Education. Adv Mater. 2019 Oct;31(43):e1904495.(IF: 30.849)

引用产品: Mouse TNF-α、IL-6、IL-12p40 and IFN-γ ELISA Kits

5. Li CX, Zhang Y, etc Artificially Reprogrammed Macrophages as Tumor-Tropic Immunosuppression-Resistant Biologics to Realize Therapeutics Production and Immune Activation. Adv Mater. 2019 Apr;31(15):e1807211.(IF: 30.849)

引用产品: Mouse TNF-α ELISA Kit