For normal cells, the content of DNA is changed with the process of cell cycle. Observed DNA stained by dyes using flow cytometry to calculate percentage of G0 /G1, S, and G2 /M. It will be clear known that how about the distribution of cell cycle and the activity of proliferation. For apoptotic cells, DNAs in cells is degraded by endogenous nuclease activated and diffuse out of cells with the process of apoptosis.

A highly definable sub-G1 peak occurs and is easily quantified by dyes. The change of DNA in apoptotic cells is also assayed for sorting and further analyzing apoptotic cells. After RNA is degraded by RNase, the nucleic acid dye in this kit bind with DNA composed of chromatin in the nucleus. And the results can be analyzed by flow cytometry.

7-AAD Cell Cycle Analysis Kit (with RNase) is used for detecting the different stages of cell cycle.

Table 1: Reagents of the Kit

KEY FEATURES

1. Easy to perform: simple and rapid procedure to perform.

2. Versatile: this kit is used for detecting cell cycle not only suspension cells but also adherence cells

3. Direct quantitation for normal, apoptotic, and dead cells by flow cytometry.

4. Ready to use

5. Highly competitive price

This kit remains stable for at least 18 months if stored at 4℃and protected from light.