Overview
| Product name | PI细胞周期检测试剂盒(含RNase) |
| Catalog no | FXP0211-200 |
| Size | 200 Tests |
Applications
| 相关介绍 | 碘化丙啶(Propidium，简称 PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光，并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后，可以用流式细胞仪对细胞进行DNA含量测定，然后根据DNA含量的分布情况，可以进行细胞周期分析。碘化丙啶染色后，假设 G0/G1 期细胞的荧光强度为1，那么含有双份基因组DNA的G2/M 期细胞的荧光强度的理论值为2，正在进行 DNA 复制的S期细胞的荧光强度为 1-2 之间。 本试剂盒通常应用于培养的贴壁或悬浮细胞的细胞周期检测。如果用于组织的细周期检测，则必须把组织消化成单细胞状态，才可以进行。 本试剂盒每个样品的细胞数量可以为10-100万。 |
| 储存条件 | 碘化丙啶染色液(25X)需避光保存。染色缓冲液和碘化丙啶溶液(25X) 4℃保存，RANase -20℃保存。12 个月内有效。 |
| 试剂盒组份 | 使用说明： 1. 细胞样品的准备： a. 对于贴壁细胞：小心收集细胞培养液到一离心管内备用。用胰酶消化细胞，至细胞可以被轻轻用移液管或枪头吹打下来时，加入前面收集的细胞培养液，吹打下所有的贴壁细胞，并轻轻吹散细胞。再次收集到离心管内。300～400g离心3-5分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充分，可以适当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的培养液，以避免吸走细胞。加入约1毫升冰浴预冷的PBS，重悬细胞，再次离心沉淀细胞，小心吸除上清。再次加入1毫升冰浴预冷的PBS，重悬细胞。 b. 对于悬浮细胞：300～400g离心3-5 分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充分，可以适当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的培养液，以避免吸走细胞。加入约1毫升冰浴预冷的PBS，重悬细胞，并转移到1.5毫升离心管内。再次离心沉淀细胞，小心吸除上清，可以残留约50微升左右的PBS，以避免吸走细胞。再次加入1毫升冰浴预冷的PBS，重悬细胞。 2. 细胞固定：取4毫升冰浴预冷的95%乙醇，把1毫升细胞悬液（细胞放在冰上）逐滴加到乙醇溶液里，充分混匀后4℃固定2小时或更长时间。固定12-24小时可能效果更佳。300～400g离心3-5 分钟，沉淀细胞。对于特定的细胞，如果细胞沉淀不充分，可以适当延长离心时间或稍稍加大离心力。小心吸除上清，可以残留约50微升左右的乙醇，以避免吸走细胞。加入约5毫升冰浴预冷的PBS，重悬细胞，再次离心沉淀细胞，小心吸除上清，可以残留约50微升左右的PBS，以避免吸走细胞。轻轻弹击离心管底以适当分散细胞，避免细胞成团。 1. 碘化丙啶染色液的配制： 参考下表，根据待检测样品的数量配制适量的碘化丙啶染色液： 注：配制好的碘化丙啶染色液短时间内可以4℃保存，宜当日使用。 4. 染色：每管细胞样品中加入0.4 毫升碘化丙啶染色液，缓慢并充分重悬细胞沉淀，37℃避光温浴30分钟。随后可以4℃或冰浴避光存放。 5. 加入2ml PBS，300～400g离心5 分钟，去上清； 6. 加入500ul的PBS，用流式细胞仪上机检测。（注：24小时内完成流式检测） 7. 流式检测和分析：用流式细胞仪在激发波长488nm波长处检测红色荧光，同时检测光散射情况。采用适当分析软件进行细胞DNA 含量分析和光散射分析。 数据分析 1、选中目的细胞群，去除碎片。 2、通过PI通道上的面积（PI-A）和高（PI-H）把粘连的细胞去除。这一步也可以用PI通道上的宽（PI-W）和高（PI-H）把粘连的细胞去除。 3、流式上可以看到PI通道荧光信号看到G0/G1、S及G2的大体位置。 4、选用分析周期的软件进行数据拟合，下图中显示了周期软件分析后的结果。 |
| 注意事项 | 1. 一般取细胞生长至覆盖率约为80%时（确保细胞未进入生长平台期）进行实验，但细胞生长密度过高,会出现接触抑制,可导致G2/M期缺失现象出现。 2. 贴壁细胞的消化时间要控制好，可边消化边观察，获得单个细胞，尽量避免DNA的降解，细胞消化固定后不可过度吹打，防止细胞碎片。 3. 为保证上机细胞数足够，染色前要进行细胞计数，保证细胞数与染料浓度匹配，避免样本管间差异较大。一般来说细胞浓度控制在2*105-2*106 个细胞/毫升。并确保细胞数和固定剂量和染料的浓度相匹配。 4. 使用离心机时，离心300～400g，且动作尽量轻柔，切记不要用力吹打细胞，尽量在4℃下操作，以免影响细胞状态。 5. 醇类固定后的样品可以在-20 °C存放几个星期，若短时间可置于4 °C避光保存。 6. 上机前，细胞过200目筛网，尽量除去细胞团。 7. 上样速度不宜过快，细胞浓度不宜过高，一般选择低速收细胞, 200个细胞/秒为佳。 8. 收细胞目的细胞20000个以上的目的细胞。 见下图E2门： 1. DNA染料，尤其是PI，具有较强的粘性。样本上机检测之后，应遵照仪器说明书仔细清洗流式细胞仪，以免对下一位操作者结果造成影响 。 2. 通过电压脉冲信号的宽度和面积区别实体组织标本中的粘连细胞群体，最大程度的减少粘连细胞带来的假阳性结果。 |
image of PI细胞周期检测试剂盒(含RNase)
PI细胞周期检测试剂盒(含RNase A)
PI细胞周期检测试剂盒(含RNase A)
PI细胞周期检测试剂盒(含RNase A)
PI细胞周期检测试剂盒(含RNase A)
| Article title: | Role of CD5 molecular-like on hepatocellular carcinoma |
| periodical: | Chinese Medical Journal |
| Journal impact factors: | 6.133 |
| Author: | Zhaowei Gao , Xianan Wu , Lan Yang.etc |
| Author Unit: | 空军军医大学唐都医院 |
| Published Time: | 2023 |
| Original link: | https://pubmed.ncbi.nlm.nih.gov/36939243/ |
| Article title: | Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical Pharmacology Available online 18 February 2021, 114475. |
| periodical: | Biochemical Pharmacology |
| Journal impact factors: | 4.96 |
| Author: | Xiaocong Xiang, Yunhong Tian, Jiani Hu, etc. |
| Author Unit: | 川北医学院南充市中心医院组织工程与干细胞研究所 |
| Published Time: | 2021 |
| Original link: | https://www.sciencedirect.com/science/article/pii/S000629522100071X |
| Article title: | Cytotoxic Nitrogenated Azaphilones from the Deep-Sea-Derived Fungus Chaetomium globosum MP4-S01-7. |
| periodical: | Journal of Natural Products |
| Journal impact factors: | 4.257 |
| Author: | Weiyi Wang, Jing Yang, Yan-Yan Liao, etc. |
| Author Unit: | 自然资源部第三海洋研究所海洋生物遗传资源重点实验室 |
| Published Time: | 2020 |
| Original link: | https://www.ncbi.nlm.nih.gov/pubmed/32193933 |
| Article title: | BAI1 acts as a tumor suppressor in lung cancer A549 cells by inducing metabolic reprogramming via the SCD1/HMGCR module. |
| periodical: | Carcinogenesis |
| Journal impact factors: | 4.004 |
| Author: | Lei Liu, Li Chai, Jingjing Ran, etc. |
| Author Unit: | 四川大学华西医院 |
| Published Time: | 2020 |
| Original link: | https://europepmc.org/article/med/32255478 |
| Article title: | Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. |
| periodical: | Food and Chemical Toxicology |
| Journal impact factors: | 3.977 |
| Author: | Zisheng Chen, Dacheng Yang, Xinwei Jiang, etc. |
| Author Unit: | 广州医科大学附属第六医院呼吸内科，清远市人民医院 |
| Published Time: | 2019 |
| Original link: | https://www.sciencedirect.com/science/article/pii/S0278691519302534 |
| Article title: | Matrine combined with cisplatin synergistically inhibited urothelial bladder cancer cells via down-regulating VEGF/PI3K/Akt signaling pathway. |
| periodical: | Cancer Cell International |
| Journal impact factors: | 3.96 |
| Author: | Xiao-Zhong Liao, Lan-Ting Tao, Jia-Hui Liu, etc. |
| Author Unit: | 中山大学附属第一医院 |
| Published Time: | 2017 |
| Original link: | https://cancerci.biomedcentral.com/articles/10.1186/s12935-017-0495-6 |
| Article title: | Tanshinone IIA combined with cisplatin synergistically inhibits non‐small‐cell lung cancer in vitro and in vivo via down‐regulating the phosphatidylinositol 3‐kinase/Akt signalling pathway. |
| periodical: | Phytotherapy Research |
| Journal impact factors: | 3.766 |
| Author: | Xiao-Zhong Liao, Ying Gao, Sheng Huang, etc. |
| Author Unit: | 广州中医药大学第一附属医院肿瘤科 |
| Published Time: | 2019 |
| Original link: | https://onlinelibrary.wiley.com/doi/full/10.1002/ptr.6392 |
| Article title: | Rosmarinic acid reverses non‐small cell lung cancer cisplatin resistance by activating the MAPK signaling pathway. |
| periodical: | Phytotherapy Research |
| Journal impact factors: | 3.766 |
| Author: | Xiao‐Zhong Liao, Ying Gao, Ling‐Ling Sun, etc. |
| Author Unit: | 广州中医药大学第一附属医院肿瘤科 |
| Published Time: | 2020 |
| Original link: | https://onlinelibrary.wiley.xilesou.top/doi/abs/10.1002/ptr.6584 |
| Article title: | Cyclovirobuxine D inhibits colorectal cancer tumorigenesis via the CTHRC1?AKT/ERK?Snail signaling pathway. |
| periodical: | International Journal of Oncology |
| Journal impact factors: | 3.571 |
| Author: | Fengqi Jiang, Yaodong Chen, Shuo Ren, etc. |
| Author Unit: | 哈尔滨医科大学第一附属医院结直肠外科 |
| Published Time: | 2020 |
| Original link: | https://www.spandidos-publications.com/10.3892/ijo.2020.5038 |
| Article title: | Dietary Supplementation of L-Arginine and N-Carbamylglutamate Attenuated the Hepatic Inflammatory Response and Apoptosis in Suckling Lambs with Intrauterine Growth Retardation. |
| periodical: | Mediators of Inflammation |
| Journal impact factors: | 3.545 |
| Author: | Hao Zhang, Yaotian Fan, Mabrouk Elsabagh, etc. |
| Author Unit: | 扬州大学动物科学与技术学院食草动物营养代谢调控研究室 |
| Published Time: | 2020 |
| Original link: | https://www.hindawi.com/journals/mi/2020/2453537/ |
| Article title: | Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder. |
| periodical: | OncoTargets and Therapy |
| Journal impact factors: | 3.337 |
| Author: | Xiaoming Wang, Yunjin Bai, Facai Zhang, etc. |
| Author Unit: | 四川大学华西医院泌尿外科研究所 |
| Published Time: | 2020 |
| Original link: | https://www.dovepress.com/targeted-inhibition-of-p4hb-promotes-cell-sensitivity-to-gemcitabine-i-peer-reviewed-fulltext-article-OTT |
| Article title: | ZNF692 promotes colon adenocarcinoma cell growth and metastasis by activating the PI3K/AKT pathway. |
| periodical: | International Journal of Oncology |
| Journal impact factors: | 3.333 |
| Author: | Yanwei Xing, Shuo Ren, Lianjie Ai, etc. |
| Author Unit: | 哈尔滨医科大学附属第一医院结直肠外科 |
| Published Time: | 2019 |
| Original link: | https://www.spandidos-publications.com/10.3892/ijo.2019.4733 |
| Article title: | A Randomized, Double-Blind, Controlled Clinical Study on the Curative Effect of Huaier on Mild-to-Moderate Psoriasis and an Experimental Study on the Proliferation of Hacat Cells. |
| periodical: | BioMed Research International |
| Journal impact factors: | 2.583 |
| Author: | Dongqiang Su, Xuening Zhang, Likun Zhang, etc. |
| Author Unit: | 哈尔滨医科大学附属第一医院皮肤科 |
| Published Time: | 2018 |
| Original link: | https://www.hindawi.com/journals/bmri/2018/2372895/abs/ |
| Article title: | miR-27a-3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1. |
| periodical: | Oncology Letters |
| Journal impact factors: | 1.871 |
| Author: | Chang Su, Dong-Ping Huang, Jian-Wen Liu, etc. |
| Author Unit: | 复旦大学中山医院闵行分院外科 |
| Published Time: | 2019 |
| Original link: | http://www.4abio.net/OtherProducts/FXP0211-200 |
| Article title: | Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells. |
| periodical: | Molecular Medicine Reports |
| Journal impact factors: | 1.851 |
| Author: | Bo Hu, Xike Zhu, Jibin Lu, etc. |
| Author Unit: | 中国医科大学盛京医院胸外科 |
| Published Time: | 2020 |
| Original link: | https://www.spandidos-publications.com/10.3892/mmr.2020.11068 |
| Article title: | Long-term 1800MHz electromagnetic radiation did not induce Balb/c-3T3 cells malignant transformation. |
| periodical: | Electromagnetic Biology and Medicine |
| Journal impact factors: | 1.82 |
| Author: | Zhen Ding , Xiaoyong Xiang , Jintao Li, etc. |
| Author Unit: | 中国医学科学院 |
| Published Time: | 2020 |
| Original link: | https://www.tandfonline.com/doi/abs/10.1080/15368378.2020.1846194 |
| Article title: | Testis-enriched circular RNA circ-Bbs9 plays an important role in Leydig cell proliferation by regulating a CyclinD2-dependent pathway. |
| periodical: | Reproduction, Fertility and Development |
| Journal impact factors: | 1.723 |
| Author: | Minzhi Jia, Xiaoliang Li, Chuan Jiang, etc. |
| Author Unit: | 四川大学华西第二医院 |
| Published Time: | 2019 |
| Original link: | http://www.publish.csiro.au/rd/RD18474 |
| Article title: | Cinobufagin Promotes Cell Cycle Arrest and Apoptosis to Block Human Esophageal Squamous Cell Carcinoma Cells Growth via the p73 Signalling Pathway. |
| periodical: | Biological and Pharmaceutical Bulletin |
| Journal impact factors: | 1.54 |
| Author: | Xu Deng,Jiexia Sheng,Hua Liu, etc. |
| Author Unit: | 锦州医科大学中国人民武装警察部队特色医学中心研究生培训基地 |
| Published Time: | 2019 |
| Original link: | https://www.jstage.jst.go.jp/article/bpb/42/9/42_b19-00174/_article/-char/ja/ |
| Article title: | The anti-proliferation effect of triterpenoid extracted from Actinidia Chinese plant root |
| periodical: | International Journal of Clinical and Experimental Medicine |
| Journal impact factors: | 1.069 |
| Author: | Xiao-Dan Yang, Tao Han, Ning Wang, etc. |
| Author Unit: | 沈阳军区总医院人民解放军肿瘤中心肿瘤科 |
| Published Time: | 2016 |
| Original link: | http://www.ijcem.com/files/ijcem0026257.pdf |
| Article title: | Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells. |
| periodical: | Pakistan Journal of Pharmaceutical Sciences |
| Journal impact factors: | 0.804 |
| Author: | Jing-Jing Xu, Ping Huang, Yun-Ya Xiang, etc. |
| Author Unit: | 广州中医药大学药学院中药药理学系 |
| Published Time: | 2018 |
| Original link: | https://www.ncbi.nlm.nih.gov/pubmed/29386153 |
¥220.00
PI细胞周期检测试剂盒(含RNase)
规格：
货期：现货
